Rapid Quality Control Test Kits

Instructions

INSTRUCTIONS

1. Place a small amount of sample into one well of the reaction plate. [weigh out 0.1 gram of ground sample if you wish to have semi-quantitative results; see scale below.] Also place one paper disc (blank) into a separate well to serve as a control.

2. Prepare Reagent A by mixing Color Reagent to Assay Buffer in a 1:10 ratio. [i.e. 1 drop to 10 drops or 1 mL to 10 mL] Make Reagent A fresh daily or every few hours if the room temperature is above 75o F (24o C).

3. Moisten the sample and blank with 5 or more drops of freshly prepared Reagent A in well of the reaction plate. [Make sure that the sample is fully wetted and excess reagent remains: if it is all absorbed by the sample add additional reagent.]

4. Allow mixture to react at room temperature for 10 to 30 minutes, preferably in the dark. The reaction plate may be covered with to give dark conditions.

5. Any blue color is a positive test. Evaluate visually the active lipase concentration [if the sample was weighed] by the blue color according to the following scale:

Intense blue* Medium blue Light blue Trace of blue No blue color
0.1 units of lipase per 0.1 g oats
0.01 units of lipase per 0.1 g oats
0.001 units of lipase per 0.1 g oats
0.00001 units of lipase per 0.1 g oats
Less than 0.00001 units of lipase per 0.1 g oats

  • Use ground raw oat groats to establish the intense blue level.

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