OAT-CHEK II

RAPID PEROXIDASE TESTING FOR OAT “COOK” QUALITY

A second part of the shelf life and product stability equation for cooked oat products is peroxidase. It is more difficult to inactivate than lipase and trace residual activity is common. Peroxidases can also renature and regenerate part of the original enzyme activity under some conditions.

Numerous colorimetric reactions can be used to follow peroxidase denaturation and renaturation during thermal processing. We selected guaiacol as the preferred color generator for its stability and excellent sensitivity without excessive reactivity. More sensitive color reactions for peroxidase exist but they are difficult to use effectively in processing areas because of the presence of oat and other peroxidase positive dust. OATCHEK II can be correlated to lab peroxidase tests if appropriate adjustments are made for enzyme-active dusts in the processing plant environment. Consequently OATCHEK II, like OATCHEK I, can be used to establish in-house standards, to generate quality assurance data and for rapid sample comparisons.

OATCHEK II was developed to allow in-plant testing of oxidative rancidity. It does require a short extraction with distilled water (not provided) prior to the actual color reaction. Tests take only a few minutes and are easily run by production personnel without laboratory support.

We think you and your production and quality assurance staffs will be pleased with the ease and speed of OATCHEK II. The kit contains enough materials for approximately 300 determinations and comes complete (except for distilled water which must be purchased locally) with extraction vials, transfer pipets, funnels and filter papers, reaction vials, reagents, dropper bottles, graduated cylinder, wash bottle, digital timer, associated items, instructions and a carry case.

Here at last is a cost effective peroxidase test with a practical production orientation. Let OATCHEK II keep you on the competitive edge of rapid “cook quality” testing technology.